Appointments and Experience
Assistant Professor. Western Michigan University, Kalamazoo, MI, 49008-5410 (7/01 to present). Joint appointment in Biological Sciences and Chemistry Departments. Coordinator of Molecular Biotechnology-High Throughput Screening (MB-HTS) Master’s Program. Interdisciplinary research methods include molecular biology, microbial physiology, high throughput screening and biophysical chemistry.
Postdoctoral Research Fellow. The Pennsylvania State University, Dept. of Biochemistry and Molecular Biology, State College, PA. (3/97-7/01). Participated in National Science Foundation Research Training Grant Program in Microbiology and Enzymology. Characterized kinetic parameters of heterologously expressed gamma carbonic anhydrase enzyme from the archaeon Methanosarcina thermophila. Deduced novel proton transport pathway for this enzyme. Collaborated with Prof. David M. Silverman and Dr. C.-K. Tu at The University of Florida, Gainesville on stop flow kinetics and oxygen-18 isotope exchange studies of this enzyme.
Postdoctoral Research Fellow. Genetics Institute, Inc., Molecular Biology and Gene Expression group, Cambridge, MA. (3/94-3/97). Investigated controlled protein affinity concept with random peptide libraries, monoclonal antibodies, thioredoxin fusion proteins and protein recognition mediated by metal ions. Developed novel surface plasmon resonance assay for antibody binding using in vivo biotinylation for capturing fusion proteins on streptavidin BIAcore chip. Measured interactions of thioredoxin variants with antibodies via Western blots, affinity chromatography, and BIAcore-surface plasmon resonance. Used FLITRX E. coli random-peptide library to epitope-map monoclonal antibodies and identify novel thioredoxin mutants with desired molecular recognition properties.
Postdoctoral Researcher. University of Delaware, Dept. of Chemical Engineering, Newark, DE, and E. I. Du Pont de Nemours and Co. Experimental Station, Wilmington, DE. (5/93-3/94). Investigated partitioning of bacteria and colloids in aqueous polymer (polyethylene glycol/dextran) two-phase systems. Used techniques of electrophoretic mobility, visual microscopy, conductiometric titration, polyelectrolyte titration, pycnometry (densiometry), and Coulter counting to analyze colloidal behavior of hydrophilic polystyrene particles.
Graduate Student (Ph.D. Candidate). University of Utah, Department of Chemical and Fuels Engineering, Salt Lake City, UT. (9/88-5/93). Advisors: Dr. Jules J. Magda and Dr. Joseph D. Andrade. Constructed digital image pendant drop device for measuring dynamic surface tension of aqueous solutions. Investigated effect of ions, surfactants, and temperature on adsorption rate and stability of aqueous protein solutions, via pendant drop dynamic surface tension measurements, differential scanning calorimetry, chromatography, UV spectroscopy.
| Undergraduate Institution
|| Colorado School of Mines, Golden, CO
|| Chemical and Petroleum Refining Engineering
|| B. Sc., 1988
| Graduate Institution
|| University of Utah, Salt Lake City, UT
|| Chemical and Fuels Engineering
|| Ph.D., 1993
| Postdoctoral Institution
||University of Delaware, Newark, DE & E. I. DuPont de Nemours, Wilmington, DE
|| Microbiology, Colloid and Surface Science
|| Genetics Institute, Inc., Cambridge, MA
|| Molecular Biology, Immunology, Protein Chemistry
||The Pennsylvania State University,University Park, PA
|| Microbial Physiology, Enzymology, Protein Folding, Biophysical Chemistry