Kane Movie Site

The movies are here, for viewing and downloading.

Generally, for a presentation or for some educational purpose, use them freely. They may not be part of a product for sale.

 

 

The Ski Hat Epiboly Movie.

 

The infamous hat that was used to explain epiboly to Developmental Biology students at University of Rochester.

Note: the black head band is supposed to be the involuting mesoderm...

 

The idea of using a cap or cup to explain epiboly I first heard from Chuck Kimmel, my mentor at Oregon. He just didn't have a stocking ski cap (that I can actually see through, by the way.)

 

This was the first thing I could think of doing with the first digital camera I ever saw.

Go figure.

 

download (mp4, 360x426, 1.6 Mb)

 

Blood Movie.

Blood over the yolk of a 1 day zebrafish.

 

This is the movie that Rachel Warga made from which she grabed pictures for the 2009 Dev Cell paper.

 

download (mp4, 640x480, 8.2 Mb)

The next three movies are supplementary data for Figure 7 in the Kane et al 2005 paper. In all cases the movies show a recording of a region of the lateral epiblast over 20 minutes from ~70-75% epiboly showing the behavior of a cell (blue) relative to a cohort of exterior layer cells (turquoise).

 

Movie 1.

Radial intercalation in ZMD heterozygote. As the interior layer cell enters the exterior layer the cohort spreads apart, causing a net gain of surface area. One of the exterior layer cells also undergoes a division, and then one of the daughters skips in and out of the interior layer (under an exterior layer cell) and then rejoins the exterior layer.

 

download (mp4, 640x480, 0.5 Mb)

 

 

 

Movie 2.

Radial intercalation in homozygous hab mutant. As the interior layer cell enters the exterior layer the cohort spreads apart, causing a net gain of surface area. From this surface view, cells in the exterior layer of the epiblast of the mutant appear smaller and more rounded than the cells in the exterior layer of the heterozygote in Movie 1.

 

download (mp4, 640x480, 0.5 Mb)

 

Movie 3.

Radial de-intercalation in homozygous hab mutant. The cells move into the interior layer (blue) (see Fig. 7C). As the cells descend, neighboring cells of the cohort close over the gap, covering the descending cells and causing a net loss of cells in the exterior layer.

 

download (mp4, 640x480, 0.5 Mb)