Confocal LSM

 

This is a brief description our confocal capabilities...

Key Benefits

bulletHigh magnification
bulletHigh resolution
bulletLarge depth of field

Specifications

bulletPlan-neo 10x/0.3, working distance 5.6mm
bulletPlan-neo 20x/0.5, working distance 2.0mm
bulletPlan-neo 40x/1.3 Oil, working distance 0.2mm
bulletC-apochromat 63x/1.2 W.corr, working distance 0.24mm
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Plan-neo 100x/1.3 Oil, working distance 0.08mm

Description of the confocal

In the Confocal/Laser Scanning Microscopy (LSM), a laser light beam is expanded to make optimal use of the optics in the objective. Through a x-y deflection mechanism this beam is turned into a scanning beam, focused to a small spot by an objective lens onto a fluorescent specimen. The mixture of reflected light and emitted fluorescent light is captured by the same objective and (after conversion into a static beam by the x-y scanner device) is focused onto a photo-detector (photomultiplier) via a dichroic mirror (beam splitter). The reflected light is deviated by the dichroic mirror while the emitted fluorescent light passes through in the direction of the photomultiplier. A confocal aperture (pinhole) is placed in front of the photo-detector, such that the fluorescent light from  the specimen that is not within the focal plane where the laser beam was focused will be largely obstructed by the pinhole. In this way, out-of-focus information (both above and below the focal plane) is greatly reduced. This becomes especially important when dealing with thick specimens. The spot that is focused on the center of the pinhole is often referred to as the "confocal spot."

Our Zeiss Axiovert 100M inverted confocal is equipped with 3 filter sets for simultaneous or individual viewing of up to three fluorescent channels. The scope is also equipped with a transmitted light source which can be viewed alone or in concert with fluorescence. Samples can be captured as individual "slices", Z-stacks or 3D rotating animations depending on your specific requirements

Sample Images

  

Capabilities

bulletThree confocal channels for reflected light, plus one for transmitted light.
bulletAll three channels can be recorded and displayed simultaneously or separately.
bulletMultitracking scanning can efficiently prevent the cross talk between different channels.
bulletImage processing functions include individual Z sections, standard 2D/3D, 3D reconstruction capabilities and 3D measurement functions as well as Z-stack and rotating 3D animations.

 

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Last modified: 01/11/05