Widefield Fluorescence Workstation

 

This is a brief description of our widefield fluorescence microscopy...

Key Benefits

bulletMorphological Identification through Differential Interference Contrast (DIC)
bulletCapable of observing a wide range of fluorophores
bulletDifferent images of the same area can be overlaid to give a DIC image that includes fluorescence

Specifications

Description of our widefield fluorescence workstation's capabilities

Fluorescent microscopy is a form of light microscopy in which the specimen is irradiated at wavelengths that excite fluorochromes. Fluorescence is used to detect structures, molecules or proteins within the cell. Fluorescent molecules absorb light at one wavelength and emit light at another, longer wavelength. When fluorescent molecules absorb a specific absorption wavelength for an electron in a given orbital, the electron rises to a higher energy level (the excited) state. Electrons in this state are unstable and will return to the ground state, releasing energy in the form of light and heat. This emission of energy is fluorescence. Because some energy is lost as heat, the emitted light contains less energy and therefore is a longer wavelength than the absorbed (or excitation) light.

In fluorescence microscopy, a sample is labeled and the label is illuminated with filtered light at the absorbing wavelength; the light emitted from the dye is viewed through a filter that allows only the emitted wavelength to be seen. The dye glows brightly against a dark background because only the emitted wavelength is allowed to reach the eyepieces or camera port of the microscope. Sometimes the fluorescent molecule itself is a direct stain or probe for specific structures. In other situations the fluorescent dye is bound to another non-fluorescent probe that recognizes specific structures.

Our widefield workstations are outfitted with _____ filter sets. We have both upright and inverted scopes, all of which are equipped with both digital and traditional Nikon cameras. Multiple images can be taken of the same area and overlayed to produce multiple channels within a single image. Our scopes also have Differential Interference Contrast (DIC) abilities which are useful in identifying structural morphology.

Sample Images

  

  

Capabilities

bulletFITC, ... and ... filter wheels
bullet250mm resolution
bulletDigital image, photographs and time-lapse video capture

 

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Last modified: 01/11/05