Catalase and Porphyrin Detection Procedure:


Catalase Test

Materials:
Bacterial culture 24-48 hrs old, 3-5% H2O2
Procedure:
A. On a plate/slant
    1.    To a plate or slant add 2 drops of H2O2
    2.    Observe for the evolution of bubbles (catalase positive reaction)

B. On a slide
    1.    Pick a colony from a plate with a wooden applicator stick (do not use a loop)
    2.    Smear colony on a clean, dry slide and apply 5 drops of H2O2
    3.    Observe for the evolution of bubbles (catalase positive reaction)

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Lab Procedures



Prophyrin Test

Materials:
Bacterial culture 24-48 hrs. old, Benzidine reagent, 3-5% H2O2
A.  On a plate/slant

    1.    To a plate or slant add 1 ml of Benzidine reagent
    2.    Now add 1 ml of 3-5% H2O2
    3.    Observe for  the development of a blue color (positive porphyrin test)

B.  On filter paper

    1.    Pick a colony from a plate with a wooden applicator stick (do not use loop)
    2.    Add 1 ml Benzidine reagent
    3.    Add 1 ml 3-5% H2O2
    4.    Observe for the development of a blue color (positive prophyrin test)

Go To Results of Porphyrin Test (Not yet available)
Lab Procedures