2. Heat fix the smears.
3. Cover the smears with a piece of absorbent paper cut to fit
the slide and place the slide on a wire gauze on a ring stand.
4. Saturate the paper with malachite green and holding the Bunsen
burner in the hand heat the slide until steam can be seen rising from the
surface. Remove the heat and reheat the slide as needed to keep the
slide steaming for about three minutes. As the paper begins to dry
add a drop or two of malachite green to keep it moist, but don't add so
much at one time that the temperature is appreciably reduced. DO
NOT OVERHEAT. The process is steaming and not baking.
5. Remove the paper with tweezers and rinse the slide thoroughly with tap water.
6. Drain the slide and counterstain 45 seconds with 0.5% safranin.
7. Wash, blot, and examine.
8. The vegetative cells will appear red and the spores will appear green.