Litmus Milk Test


Purpose:  To differentiate bacteria based on various reactions that occur in skim milk suplemented with a litmus pH indicator.

Principle:  Milk is a complex nutritional source that contains proteins (mainly casein) in an aqueous solution of lactose and minerals.  Bacterial enzymes alter the media and may bring about various changes.  Litmus is added to the medium to detect pH changes that may occur as a result of these enzymatic reactions.  Above a pH of 8.3 litmus is blue, while below a pH of 4.5 litmus is red.

Possible Results:

A.  Acid/Reduction/Curd
B.  Reduction/Curd (arrow denotes gas pocket)
C.  Uninoculated Control
D.  Acid Formation
E.  Proteolysis of casein
F.  Alkaline Reaction

By producing acid from the fermentation of lactose present in milk a bacterium may also cause the milk to curdle or clot in the bottom of the tube.  Litmus may also act as an electron acceptor thus becoming reduced by bacterial metabolism.  This reaction is observed as a white color in the medium.

The photo at the right illustrates clot formation and redution of litmus. 

A.  Represents a tube that produced a curd and reduced litmus. 

B.  Represents an uninoculated control.

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